CE Certification Malaria Pf Factory Quotes – Novel Coronavirus (2019-nCoV) Nucleic Acid Test Kit (Multiple Fluorescence PCR) – INNOVITA
CE Certification Malaria Pf Factory Quotes – Novel Coronavirus (2019-nCoV) Nucleic Acid Test Kit (Multiple Fluorescence PCR) – INNOVITA Detail:
Product Detail:
Innovita® 2019-nCoV IgM/IgG Test is intended for the diagnosis and epidemiological monitoring of diseases caused by the novel coronavirus (2019-nCoV). ORF1ab and N gene of 2019-nCoV are detected qualitatively from throat swabs and alveolar lavage fluid samples collected from suspected cases of pneumonia, patients with suspected cases, others who need to diagnose.
The test results of this kit are for clinical reference only. It is recommended to conduct a comprehensive analysis of the condition based on the patient’s clinical manifestations and other laboratory tests.
Principle:
This kit uses one-step reverse transcription polymerase chain reaction (RT-PCR) detection technology to target the novel coronavirus (2019-nCoV) ORF1ab gene, N gene, and human internal reference gene sequence. Specific primers and taqman probes were designed in conserved regions.
Composition:
|
Composition |
48 tests / kit |
| Reaction Mix A | 792μL×1Tube |
| Reaction Mix B | 168μL×1Tube |
| Positive Control | 50μL×1Tube |
| Negative Control | 50μL ×1Tube |
Note: 1. Different batches of reagents should not be mixed.
2. Positive controls and positive controls need not be extracted
Test Procedure:
1. Nucleic acid extraction:
Commercial RNA extraction kits are available, magnetic bead extraction and spin column extraction are recommended for this kit.
2. Preparation the Reaction Mix:
● Take out the 2019-nCoV reaction Mix A/B and keep at room temperature until unfreezing;
● Take the corresponding portions (Reaction Mix A 16.5μL/T, Reaction Mix B 3.5μL/T) and mix, and then aliquot each PCR reaction with 20μL/ tube;
● Add 5μL of RNA template or negative control or positive control, then cover the tube cap;
● Place the reaction tube in the fluorescence PCR instrument, and set the negative / positive control and sample parameters for RT-PCR reaction according to the instrument operating instructions.
● Record Sample placement order
3.RT-PCR protocols:
Recommended Settings:
|
Cycle |
Time |
Temperature（℃） |
|
|
1 |
1 |
10min |
25 |
|
2 |
1 |
10min |
50 |
|
3 |
1 |
10min |
95 |
|
4 |
45 |
10s |
95 |
|
35s |
55 |
Product detail pictures:
Related Product Guide:
Our staff are usually in the spirit of "continuous improvement and excellence", and while using the top-quality high-quality items, favorable value and superior after-sales services, we try to acquire each and every customer's belief for CE Certification Malaria Pf Factory Quotes – Novel Coronavirus (2019-nCoV) Nucleic Acid Test Kit (Multiple Fluorescence PCR) – INNOVITA , The product will supply to all over the world, such as: Hamburg, Irish, Bangladesh, Our staffs are rich in experience and trained strictly, with professional knowledge, with energy and always respect their customers as the No. 1, and promise to do their best to provide the effective and individual service for customers. The Company pays attention to maintaining and developing the long-term cooperation relationship with the customers. We promise, as your ideal partner, we will develop a bright future and enjoy the satisfying fruit together with you, with persisting zeal, endless energy and forward spirit.
By Erica from Durban - 2017.06.19 13:51
It is a very good, very rare business partners, looking forward to the next more perfect cooperation!
By Judy from Uganda - 2018.06.21 17:11
