China One Step Saliva Rapid Test Kit Factory Quotes – Novel Coronavirus (2019-nCoV) Nucleic Acid Test Kit (Multiple Fluorescence PCR) – INNOVITA
China One Step Saliva Rapid Test Kit Factory Quotes – Novel Coronavirus (2019-nCoV) Nucleic Acid Test Kit (Multiple Fluorescence PCR) – INNOVITA Detail:
Product Detail:
Innovita® 2019-nCoV IgM/IgG Test is intended for the diagnosis and epidemiological monitoring of diseases caused by the novel coronavirus (2019-nCoV). ORF1ab and N gene of 2019-nCoV are detected qualitatively from throat swabs and alveolar lavage fluid samples collected from suspected cases of pneumonia, patients with suspected cases, others who need to diagnose.
The test results of this kit are for clinical reference only. It is recommended to conduct a comprehensive analysis of the condition based on the patient’s clinical manifestations and other laboratory tests.
Principle:
This kit uses one-step reverse transcription polymerase chain reaction (RT-PCR) detection technology to target the novel coronavirus (2019-nCoV) ORF1ab gene, N gene, and human internal reference gene sequence. Specific primers and taqman probes were designed in conserved regions.
Composition:
|
Composition |
48 tests / kit |
| Reaction Mix A | 792μL×1Tube |
| Reaction Mix B | 168μL×1Tube |
| Positive Control | 50μL×1Tube |
| Negative Control | 50μL ×1Tube |
Note: 1. Different batches of reagents should not be mixed.
2. Positive controls and positive controls need not be extracted
Test Procedure:
1. Nucleic acid extraction:
Commercial RNA extraction kits are available, magnetic bead extraction and spin column extraction are recommended for this kit.
2. Preparation the Reaction Mix:
● Take out the 2019-nCoV reaction Mix A/B and keep at room temperature until unfreezing;
● Take the corresponding portions (Reaction Mix A 16.5μL/T, Reaction Mix B 3.5μL/T) and mix, and then aliquot each PCR reaction with 20μL/ tube;
● Add 5μL of RNA template or negative control or positive control, then cover the tube cap;
● Place the reaction tube in the fluorescence PCR instrument, and set the negative / positive control and sample parameters for RT-PCR reaction according to the instrument operating instructions.
● Record Sample placement order
3.RT-PCR protocols:
Recommended Settings:
|
Cycle |
Time |
Temperature（℃） |
|
|
1 |
1 |
10min |
25 |
|
2 |
1 |
10min |
50 |
|
3 |
1 |
10min |
95 |
|
4 |
45 |
10s |
95 |
|
35s |
55 |
Product detail pictures:
Related Product Guide:
We constantly continually give you essentially the most conscientious client provider, plus the widest variety of designs and styles with finest materials. These initiatives include the availability of customized designs with speed and dispatch for China One Step Saliva Rapid Test Kit Factory Quotes – Novel Coronavirus (2019-nCoV) Nucleic Acid Test Kit (Multiple Fluorescence PCR) – INNOVITA , The product will supply to all over the world, such as: Bolivia, Salt Lake City, Sweden, Our solutions have national accreditation requirements for qualified, good quality products, affordable value, was welcomed by individuals all over the world. Our products will continue to improve inside the order and appear forward to cooperation with you, Definitely should any of those goods be of curiosity to you, be sure to letus know. We'll be satisfied to supply you a quotation up on receipt of the detailed needs.
By Andrew Forrest from Turkey - 2018.06.30 17:29
It is not easy to find such a professional and responsible provider in today's time. Hope that we can maintain long-term cooperation.
By Gill from Senegal - 2017.10.27 12:12
