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Product Detail:

Innovita® 2019-nCoV IgM/IgG Test is intended for the diagnosis and epidemiological monitoring of diseases caused by the novel coronavirus (2019-nCoV). ORF1ab and N gene of 2019-nCoV are detected qualitatively from throat swabs and alveolar lavage fluid samples collected from suspected cases of pneumonia, patients with suspected cases, others who need to diagnose.
The test results of this kit are for clinical reference only. It is recommended to conduct a comprehensive analysis of the condition based on the patient’s clinical manifestations and other laboratory tests.

Principle:

This kit uses one-step reverse transcription polymerase chain reaction (RT-PCR) detection technology to target the novel coronavirus (2019-nCoV) ORF1ab gene, N gene, and human internal reference gene sequence. Specific primers and taqman probes were designed in conserved regions.

Composition:

Note: 1. Different batches of reagents should not be mixed.
2. Positive controls and positive controls need not be extracted

Test Procedure:

1. Nucleic acid extraction:
Commercial RNA extraction kits are available, magnetic bead extraction and spin column extraction are recommended for this kit.
2. Preparation the Reaction Mix:

● Take out the 2019-nCoV reaction Mix A/B and keep at room temperature until unfreezing;
● Take the corresponding portions (Reaction Mix A 16.5μL/T, Reaction Mix B 3.5μL/T) and mix, and then aliquot each PCR reaction with 20μL/ tube;
● Add 5μL of RNA template or negative control or positive control, then cover the tube cap;
● Place the reaction tube in the fluorescence PCR instrument, and set the negative / positive control and sample parameters for RT-PCR reaction according to the instrument operating instructions.
● Record Sample placement order

3.RT-PCR protocols:

Recommended Settings:

The client fulfillment is our primary concentrate on. We uphold a consistent level of professionalism, excellent, credibility and service for Low price for China Breathable Hot Sale Polyester Highlight Reflective Safety Vest, The principle of our business will be to give high-quality products, expert provider, and trustworthy communication. Welcome all pals to place trial get for making a long-term business enterprise connection.
Low price for China Reflective Safety Vest and Breathable Reflective Safety Vest price, Our solutions have national accreditation requirements for qualified, good quality goods, affordable value, was welcomed by individuals all over the world. Our goods will continue to improve inside the order and appear forward to cooperation with you, Seriously should any of individuals items be of interest for you, please letus know. We are going to be satisfied to provide you a quotation up on receipt of the comprehensive needs.