Wholesale Uncut Sheet Kit Factory Quotes – Novel Coronavirus (2019-nCoV) Nucleic Acid Test Kit (Multiple Fluorescence PCR) – INNOVITA Manufacturer and Supplier

Uncut Sheet Kit Factory Quotes – Novel Coronavirus (2019-nCoV) Nucleic Acid Test Kit (Multiple Fluorescence PCR) – INNOVITA

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Uncut Sheet Kit Factory Quotes – Novel Coronavirus (2019-nCoV) Nucleic Acid Test Kit (Multiple Fluorescence PCR) – INNOVITA Detail:

Product Detail:

Innovita® 2019-nCoV IgM/IgG Test is intended for the diagnosis and epidemiological monitoring of diseases caused by the novel coronavirus (2019-nCoV). ORF1ab and N gene of 2019-nCoV are detected qualitatively from throat swabs and alveolar lavage fluid samples collected from suspected cases of pneumonia, patients with suspected cases, others who need to diagnose.
The test results of this kit are for clinical reference only. It is recommended to conduct a comprehensive analysis of the condition based on the patient’s clinical manifestations and other laboratory tests.

Principle:

This kit uses one-step reverse transcription polymerase chain reaction (RT-PCR) detection technology to target the novel coronavirus (2019-nCoV) ORF1ab gene, N gene, and human internal reference gene sequence. Specific primers and taqman probes were designed in conserved regions.

Composition:

Composition	48 tests / kit
Reaction Mix A	792μL×1Tube
Reaction Mix B	168μL×1Tube
Positive Control	50μL×1Tube
Negative Control	50μL ×1Tube

Note: 1. Different batches of reagents should not be mixed.
2. Positive controls and positive controls need not be extracted

Test Procedure:

1. Nucleic acid extraction:
Commercial RNA extraction kits are available, magnetic bead extraction and spin column extraction are recommended for this kit.
2. Preparation the Reaction Mix:

● Take out the 2019-nCoV reaction Mix A/B and keep at room temperature until unfreezing;
● Take the corresponding portions (Reaction Mix A 16.5μL/T, Reaction Mix B 3.5μL/T) and mix, and then aliquot each PCR reaction with 20μL/ tube;
● Add 5μL of RNA template or negative control or positive control, then cover the tube cap;
● Place the reaction tube in the fluorescence PCR instrument, and set the negative / positive control and sample parameters for RT-PCR reaction according to the instrument operating instructions.
● Record Sample placement order

3.RT-PCR protocols:

Recommended Settings:

	Cycle	Time	Temperature（℃）
1	1	10min	25
2	1	10min	50
3	1	10min	95
4	45	10s	95
4	45	35s	55

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